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KMID : 0363320100310010084
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Journal of Korean Oriental Internal Medicine
2010 Volume.31 No. 1 p.84 ~ p.101
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Protective Effects of Seonpyejeongcheon-tang on Elastase-Induced Lung Injury in Mice
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Yoon Jong-Man
Park Yang-Chun
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Abstract
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Objectives : This study aimed to evaluate the protective effects of Seonpyejeongcheon-tang (SJT) on elastase-induced lung injury.
Materials and Methods : The extract of SJT was treated to A549 cells and an elastase-induced lung injury mouse model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed.
Results : SJT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of cyclin B 1, Cdk1, and Erk1/2, and gene expression of TNF- α and IL- 1β in A549 cells. SJT treatment also revealed a protective effect on elastase-induced lung injury in mouse model. This effect was evidenced via histopathological findings, including immunofluoresence stains against elastin, collagen, and caspase 3, and protein levels of cyclin B1, Cdc2, and Erk1/2 in lung tissue.
Conclusion : These data suggest that SJT has pharmaceutical properties on lung injury. This study thus provides scientific evidence for the efficacy of SJT for clinical application to patients with chronic obstructive pulmonary disease.
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KEYWORD
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Seonpyejeongcheon-tang (Xuanfeidingchuan-tang), Chronic obstructive pulmonary disease, Emphysema, A549 cell, Elastase
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